High-speed, large field-of-view and deep imaging with an adaptive excitation source

Bo Li; Chunyan Wu; Mengran Wang; Kriti Charan; Chris Xu

doi:10.1117/12.2549312

14 February 2020 High-speed, large field-of-view and deep imaging with an adaptive excitation source

Bo Li, Chunyan Wu, Mengran Wang, Kriti Charan, Chris Xu

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112442L (2020) https://doi.org/10.1117/12.2549312
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Conventional multiphoton microscopy uses periodically pulsed sources as excitation and the sample is illuminated uniformly by the laser. While necessary for structural imaging, monitoring dynamic biological functions such as neuronal activity in the brain typically only requires imaging of the region of interest (ROI), e.g., the neurons. The adaptive excitation source enables imaging of the region of interest only. It reduces the requirement for the output power of the excitation source (by at least an order of magnitude) and simultaneously reduces the excitation power to the sample for obtaining the necessary information (e.g., neuronal activity). We demonstrate three-photon imaging of brain activity in awake transgenic mice (jRGECO1a), with highest speed (30 frames/s), large field-of-view (620x620 μm/512x512 pixels) and deep penetration (750 μm beneath the dura).

Citation Download Citation

Bo Li, Chunyan Wu, Mengran Wang, Kriti Charan, and Chris Xu "High-speed, large field-of-view and deep imaging with an adaptive excitation source", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112442L (14 February 2020); https://doi.org/10.1117/12.2549312

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