Multifocus imaging via polarization wavefront shaping

Chen Li; Changqin Ding; Minghe Li; Jiayue Rong; James Ulcickas; Garth J. Simpson

doi:10.1117/12.2546543

17 February 2020 Multifocus imaging via polarization wavefront shaping

Chen Li, Changqin Ding, Minghe Li, Jiayue Rong, James Ulcickas, Garth J. Simpson

Proceedings Volume 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII; 112450T (2020) https://doi.org/10.1117/12.2546543
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Conventional three-dimensional (3D) images of biological samples are typically assembled from a stack of twodimensional images acquired sequentially at different focal planes. This time-consuming manner hinders the application of 3D imaging techniques to the investigation of fast biochemical dynamics and light-sensitive biological events. The concept of multifocus imaging, which enables simultaneous acquisition of images from multiple focal planes, was introduced to achieve rapid 3D imaging. In the present study, we achieved multifocus imaging through polarization wavefront shaping via a micro-retarder array which splits the incident linearly polarized light into three beamlets that are focused to three axially-offset focal planes with ~100 μm separation. Append to an existing beam-scanning microscope, this multifocus system enables rapid 3D imaging compatible with a variety of optical microscopic approaches including laser transmittance, two-photon excited fluorescence, and second harmonic generation microscopy.

Conference Presentation

Citation Download Citation

Chen Li, Changqin Ding, Minghe Li, Jiayue Rong, James Ulcickas, and Garth J. Simpson "Multifocus imaging via polarization wavefront shaping", Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 112450T (17 February 2020); https://doi.org/10.1117/12.2546543

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available