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9 March 2020 Fluorescence lifetime image scanning microscopy (Conference Presentation)
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Abstract
Image Scanning Microscopy (ISM) enhances the spatial resolution of a confocal microscope by simple means. An extension to realize fluorescence lifetime imaging in combination with ISM seems straight-forward. First realizations have been reported by Israel et al. and Castello et al. [1,2] Here, we present a cost-efficient detector scheme based on a commercial multi-anode PMT that allows to perform fluorescence lifetime imaging microscopy (FLIM) in combination with ISM. We developa dedicated amplification electronics that allows for counting signals from 32 detector pixels using a commercial eight channel TCSPC hardware. [1] Y. Israel, R. Tenne, D. Oron, and Y. Silberberg “Quantum correlation enhanced super-resolution localization microscopy enabled by a fibre bundle camera” Nat. Commun. 8 (2017) 14786. [2] M. Castello et al. “A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM” Nat. Methods 16 (2019) 175-178.
Conference Presentation
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Ingo Gregor, Niels Radmacher, and Jörg Enderlein "Fluorescence lifetime image scanning microscopy (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124606 (9 March 2020); https://doi.org/10.1117/12.2546532
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