Ultrafast fluorescence lifetime imaging microscopy by frequency-division multiplexing (Conference Presentation)

Hiroshi Kanno; Hideharu Mikami; Keisuke Goda

doi:10.1117/12.2550427

26 March 2020 Ultrafast fluorescence lifetime imaging microscopy by frequency-division multiplexing (Conference Presentation)

Hiroshi Kanno, Hideharu Mikami, Keisuke Goda

Author Affiliations +

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460D (2020) https://doi.org/10.1117/12.2550427
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We demonstrate ultrafast fluorescence lifetime imaging microscopy (FLIM) based on frequency-division multiplexing. As a proof-of-concept demonstration, we obtained images with fluorescence intensity and lifetime contrasts of MCF-7 breast cancer cells stained by SYTO16 at a record high frame rate of 16,000 fps, which is 100 times higher than that of previously reported FLIM techniques. Our method is expected to expand the utility of FLIM to quantitative analysis of rapid intracellular dynamics and high-throughput cell screening based on fluorescence lifetime images.

Conference Presentation

Citation Download Citation

Hiroshi Kanno, Hideharu Mikami, and Keisuke Goda "Ultrafast fluorescence lifetime imaging microscopy by frequency-division multiplexing (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460D (26 March 2020); https://doi.org/10.1117/12.2550427

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available