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9 March 2020 Achieving spontaneous super-resolution in a confocal microscope by exploiting super-linear emitters (Conference Presentation)
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Abstract
We achieve spontaneous 3D super-resolution on a standard confocal microscope by exploiting bio-friendly fluorescent markers with super-linear excitation-emission dependence (upconversion nanoparticles of NaYF4: Yb, Tm). We refer to this approach as upconversion super-linear excitation-emission (uSEE) microscopy. To demonstrate the applicability of the method for biological applications, we image sugar-coated upconversion nanoparticles in neuronal cells and we achieve resolution twice better than the diffraction limit both in lateral and axial directions. We envision that due to the application simplicity of the developed methodological toolbox, uSEE microscopy can be widely incorporated as an everyday super-resolution method in biological laboratories.
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Denitza Denkova, Martin Ploschner, Minakshi Das, Lindsay M. Parker, Xianlin Zheng, Yiqing Lu, Antony Orth, Nicolle H. Packer, and James A. Piper "Achieving spontaneous super-resolution in a confocal microscope by exploiting super-linear emitters (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460H (9 March 2020); https://doi.org/10.1117/12.2544364
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