Single molecule localization with four avalanche photodiode detectors

Megan K. Dunlap; Duncan P. Ryan; Peter M. Goodwin; James H. Werner; Somak Majumder; Jennifer A. Hollingsworth; Martin P. Gelfand; Alan Van Orden

doi:10.1117/12.2546573

13 February 2020 Single molecule localization with four avalanche photodiode detectors

Megan K. Dunlap, Duncan P. Ryan, Peter M. Goodwin, James H. Werner, Somak Majumder, Jennifer A. Hollingsworth, Martin P. Gelfand, Alan Van Orden

Author Affiliations +

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460O (2020) https://doi.org/10.1117/12.2546573
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Photoluminescence images can be acquired with detection schemes that have both single-photon sensitivity and nanosecond scale temporal resolution, enabling the study of possible structural bases for photoluminescence lifetimes and other features of the photon arrival statistics. Within the context of super-resolution (SR) imaging, this has been demonstrated with detection schemes that collect images with a bundle of optical fibers that are coupled to individual single-photon counting avalanche photodiode detectors. Recently, our group used a bundle of four optical fibers to collect these “time-resolved photon arrival” images. Despite the paucity of information contained in a four-pixel image, we precisely located the emission centroid of quantum dots (QDs) and observed correlations between centroid location, photoluminescence lifetime, and intensity within clusters of QDs that were suggestive of electronic interactions among them. This proceedings paper details the approach that we used to locate the emission centroid based on the counts in the four detectors.

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Citation Download Citation

Megan K. Dunlap, Duncan P. Ryan, Peter M. Goodwin, James H. Werner, Somak Majumder, Jennifer A. Hollingsworth, Martin P. Gelfand, and Alan Van Orden "Single molecule localization with four avalanche photodiode detectors", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460O (13 February 2020); https://doi.org/10.1117/12.2546573

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