Characterization of single-molecule dwell times beyond photobleaching

Thilini Perera; Wendi Fu; Ying Hu

doi:10.1117/12.2546920

13 February 2020 Characterization of single-molecule dwell times beyond photobleaching

Thilini Perera, Wendi Fu, Ying Hu

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 1124615 (2020) https://doi.org/10.1117/12.2546920
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

In this work we report a single molecule imaging method to spread the photon budget of a fluorophore over extended time periods by inserting a non-illuminating interval between consecutive frames. While photobleaching results in loss of fluorescent signals similar to the detachment of a fluorescently labeled biomolecules, we introduced this method to characterize the dwell times of single molecules.

Citation Download Citation

Thilini Perera, Wendi Fu, and Ying Hu "Characterization of single-molecule dwell times beyond photobleaching", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124615 (13 February 2020); https://doi.org/10.1117/12.2546920

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