Sensorless and sensor based adaptive optics for light sheet microscopy

Yang Liu; Keelan Lawrence; James D. Lauderdale; Peter Kner

doi:10.1117/12.2543251

17 February 2020 Sensorless and sensor based adaptive optics for light sheet microscopy

Yang Liu, Keelan Lawrence, James D. Lauderdale, Peter Kner

Proceedings Volume 11248, Adaptive Optics and Wavefront Control for Biological Systems VI; 1124806 (2020) https://doi.org/10.1117/12.2543251
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Light Sheet Microscopy has developed rapidly over the past decade and is the ideal approach for imaging model organisms such as zebrafish and other thick tissue specimens. Despite the superior optical sectioning capability, high imaging speed, and large field of view, the performance of light sheet microscopy still suffers from optical aberrations. We have implemented a scene-based Shack-Hartmann wavefront sensor for directly measuring the optical aberrations on the emission side of the light-sheet microscope. In this work, we show that our system is capable of AO correction using sensor based and sensorless based approaches. We demonstrate correction up-to one hundred microns deep in zebrafish and fruitfly embryos.

Conference Presentation

Citation Download Citation

Yang Liu, Keelan Lawrence, James D. Lauderdale, and Peter Kner "Sensorless and sensor based adaptive optics for light sheet microscopy", Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124806 (17 February 2020); https://doi.org/10.1117/12.2543251

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