Closed-loop multiconjugate adaptive optics for microscopy

Karen M. Hampson; Jiahe Cui; Matthew Wincott; Richard Lane; Syed Hussain; Kaustubh Banerjee; Pouya Rajaeipour; Hans Zappe; Çağlar Ataman; Martin J. Booth

doi:10.1117/12.2544391

17 February 2020 Closed-loop multiconjugate adaptive optics for microscopy

Karen M. Hampson, Jiahe Cui, Matthew Wincott, Richard Lane, Syed Hussain, Kaustubh Banerjee, Pouya Rajaeipour, Hans Zappe, Çağlar Ataman, Martin J. Booth

Author Affiliations +

Proceedings Volume 11248, Adaptive Optics and Wavefront Control for Biological Systems VI; 1124809 (2020) https://doi.org/10.1117/12.2544391
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

When imaging a sample, inhomogeneities in refractive index cause blur in the image and decrease resolution. Adaptive optics (AO) is a technique that can correct for the resulting aberrations. The most common implementation of AO uses a single deformable mirror that is conjugate to the pupil. A single pupil-conjugate corrective device provides correction over a limited field of view owing to field-dependent aberrations. To overcome this limitation, an additional specimen-conjugate deformable mirror can be used. However, adding a second reflective correction device significantly increases system complexity. We have developed a closed-loop multiconjugate AO system for field-dependent aberration correction in a confocal fluorescence microscope. A 140-actuator deformable mirror is used in the pupil plane with a custom 37-element transmissive deformable phase plate inserted in a sample-conjugate plane. Both devices are calibrated and controlled in closed-loop using a Shack-Hartmann sensor in combination with an integral control law. The sensor consists of an EMCCD and lenslet array with a 500 μm pitch and a 47 mm focal length. Results from a Drosophila ovary and HeLa cells are presented.

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Karen M. Hampson, Jiahe Cui, Matthew Wincott, Richard Lane, Syed Hussain, Kaustubh Banerjee, Pouya Rajaeipour, Hans Zappe, Çağlar Ataman, and Martin J. Booth "Closed-loop multiconjugate adaptive optics for microscopy", Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124809 (17 February 2020); https://doi.org/10.1117/12.2544391

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KEYWORDS

Adaptive optics

Sensors

Wavefront sensors

Luminescence

Microscopes

Microscopy

Confocal microscopy

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