CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 11248 > Article
Paper
17 February 2020 Sensorless adaptive optics with a laser free spinning disk confocal microscope
S. A. Hussain, T. Kubo, N. Hall, K. Hampson, M. A. Phillips, D. Gala, I. M. Dobbie, J. Antonello, M. Wincott, I. Davis, M. J. Booth
Author Affiliations +
Proceedings Volume 11248, Adaptive Optics and Wavefront Control for Biological Systems VI; 112480W (2020) https://doi.org/10.1117/12.2542959
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Specimen induced aberrations can have detrimental effects in all types of high-resolution microscope. In this study, we present a sensorless technique that uses a deformable mirror (DM) to correct aberrations of both the system and sample. Using a laser-free confocal microscope, with patterned disk illumination and detection. The system is based on a commercial confocal module (Clarity, Aurox Ltd., UK) that uses Light Emitting Diode (LED) illumination to obtain optically sectioned 3D images. The results obtained show that the setup was able to correct aberrations of biological samples used in the study. These systems will help researchers working on various biological systems to obtain improved quality images when focussing deep into thick specimens.
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
S. A. Hussain, T. Kubo, N. Hall, K. Hampson, M. A. Phillips, D. Gala, I. M. Dobbie, J. Antonello, M. Wincott, I. Davis, and M. J. Booth "Sensorless adaptive optics with a laser free spinning disk confocal microscope", Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 112480W (17 February 2020); https://doi.org/10.1117/12.2542959
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 5 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Microscopes
Confocal microscopy
Adaptive optics
Wavefronts
Image quality
Light emitting diodes
Deformable mirrors
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top