Sensorless adaptive optics with a laser free spinning disk confocal microscope

S. A. Hussain; T. Kubo; N. Hall; K. Hampson; M. A. Phillips; D. Gala; I. M. Dobbie; J. Antonello; M. Wincott; I. Davis; M. J. Booth

doi:10.1117/12.2542959

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17 February 2020 Sensorless adaptive optics with a laser free spinning disk confocal microscope

S. A. Hussain, T. Kubo, N. Hall, K. Hampson, M. A. Phillips, D. Gala, I. M. Dobbie, J. Antonello, M. Wincott, I. Davis, M. J. Booth

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Proceedings Volume 11248, Adaptive Optics and Wavefront Control for Biological Systems VI; 112480W (2020) https://doi.org/10.1117/12.2542959
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Specimen induced aberrations can have detrimental effects in all types of high-resolution microscope. In this study, we present a sensorless technique that uses a deformable mirror (DM) to correct aberrations of both the system and sample. Using a laser-free confocal microscope, with patterned disk illumination and detection. The system is based on a commercial confocal module (Clarity, Aurox Ltd., UK) that uses Light Emitting Diode (LED) illumination to obtain optically sectioned 3D images. The results obtained show that the setup was able to correct aberrations of biological samples used in the study. These systems will help researchers working on various biological systems to obtain improved quality images when focussing deep into thick specimens.

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S. A. Hussain, T. Kubo, N. Hall, K. Hampson, M. A. Phillips, D. Gala, I. M. Dobbie, J. Antonello, M. Wincott, I. Davis, and M. J. Booth "Sensorless adaptive optics with a laser free spinning disk confocal microscope", Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 112480W (17 February 2020); https://doi.org/10.1117/12.2542959

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