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11 March 2020 Quantifying myelination of single neurons using Spatial Light Interference Microscopy (SLIM) (Conference Presentation)
Michael J. Fanous, Young Jae Lee, Catherine A. Best-Popescu, Allison Louie, Andrew Steelman, Megan P. Caputo, Laurie A. Rund, Rodney W. Johnson, Tapas Das, Matthew J. Kuchan, Gabriel Popescu
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Proceedings Volume 11249, Quantitative Phase Imaging VI; 112490T (2020) https://doi.org/10.1117/12.2550330
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Although both neurons and oligodendrocytes have been well studied individually, very little is known about how they interact with each other. New methods are needed to further study the intricacies of this interplay in terms of cellular and molecular dynamics. Spatial Light Interference Microscopy (SLIM) is a quantitative phase imaging technique that generates phase maps related to the dry mass content of the sample. In this work, we study the ability of SLIM to quantify myelination at the axonal level. We imaged a series of cocultures comprising hippocampal neurons and oligodendrocytes, of varying densities, using SLIM, and evaluated dry mass formation and growth of myelin.
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Michael J. Fanous, Young Jae Lee, Catherine A. Best-Popescu, Allison Louie, Andrew Steelman, Megan P. Caputo, Laurie A. Rund, Rodney W. Johnson, Tapas Das, Matthew J. Kuchan, and Gabriel Popescu "Quantifying myelination of single neurons using Spatial Light Interference Microscopy (SLIM) (Conference Presentation)", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490T (11 March 2020); https://doi.org/10.1117/12.2550330
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KEYWORDS
Neurons
Microscopy
Alzheimer's disease
Biomedical optics
Brain
Epilepsy
Nervous system
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