Fast imaging of cardiomyocyte dynamics alterations after drug treatment utilizing quantitative phase digital holographic microscopy

Björn Kemper; Steffi Ketelhut; Ivan Stojanovic; Martin L. Bennink; Peter Schön; Jürgen Schnekenburger

doi:10.1117/12.2546255

14 February 2020 Fast imaging of cardiomyocyte dynamics alterations after drug treatment utilizing quantitative phase digital holographic microscopy

Björn Kemper, Steffi Ketelhut, Ivan Stojanovic, Martin L. Bennink, Peter Schön, Jürgen Schnekenburger

Author Affiliations +

Proceedings Volume 11249, Quantitative Phase Imaging VI; 112491S (2020) https://doi.org/10.1117/12.2546255
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Digital holographic microscopy (DHM) is an interferometry-based variant of quantitative phase microscopy (QPM) that can be integrated modular into various common microscopes for label-free imaging of fast cellular morphology changes in a biomedical laboratory environment. We have utilized a fiber optics-based off-axis DHM concept to monitor the dynamics of beating cardiomyocytes after drug treatment. Our results show that local height changes due to cellular contractions can be detected spatially resolved at the subcellular level. Moreover, we demonstrate that a temporal resolution in the millisecond range is sufficient to detect drug induced increases and decreases of the beating rate.

Citation Download Citation

Björn Kemper, Steffi Ketelhut, Ivan Stojanovic, Martin L. Bennink, Peter Schön, and Jürgen Schnekenburger "Fast imaging of cardiomyocyte dynamics alterations after drug treatment utilizing quantitative phase digital holographic microscopy", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112491S (14 February 2020); https://doi.org/10.1117/12.2546255

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