2D image-guided cell sorter and 3D imaging flow cytometer

Rui Tang; Xinyu Chen; Zunming Zhang; Lauren Waller; Jiajie Chen; Yi Gu; Yuanyuan Han; Chang-Hung Lee; Ivan Gagne; Alex Ce Zhang; Sung Hwan Cho; Yu-Hwa Lo

doi:10.1117/12.2547689

20 February 2020 2D image-guided cell sorter and 3D imaging flow cytometer

Rui Tang, Xinyu Chen, Zunming Zhang, Lauren Waller, Jiajie Chen, Yi Gu, Yuanyuan Han, Chang-Hung Lee, Ivan Gagne, Alex Ce Zhang, Sung Hwan Cho, Yu-Hwa Lo

Author Affiliations +

Proceedings Volume 11250, High-Speed Biomedical Imaging and Spectroscopy V; 112500H (2020) https://doi.org/10.1117/12.2547689
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Cell type classification and isolation according to imaging and spatial characteristics, beyond traditional fluorescently labeled biomarkers, enable the development of new biological insight and establishment of connections between phenotypical, morphological, and genomic cell information in normal and diseased states. Here we demonstrate a 2D image-guided cell sorter and a 3D imaging flow cytometer using fast scanning laser excitation sources. Both systems feature a cameraless design, which reconstructs cell images from the temporal readout of photomultiplier tubes.

Conference Presentation

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Rui Tang, Xinyu Chen, Zunming Zhang, Lauren Waller, Jiajie Chen, Yi Gu, Yuanyuan Han, Chang-Hung Lee, Ivan Gagne, Alex Ce Zhang, Sung Hwan Cho, and Yu-Hwa Lo "2D image-guided cell sorter and 3D imaging flow cytometer", Proc. SPIE 11250, High-Speed Biomedical Imaging and Spectroscopy V, 112500H (20 February 2020); https://doi.org/10.1117/12.2547689

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