1Lab. des sciences de l'Ingénieur, de l'Informatique et de l'Imagerie (France) 2Institut National des Sciences Appliquées de Strasbourg (France) 3Lab. des sciences de l'Ingénieur, de l'Informatique et de l'Imagerie, CNRS (France) 4Ctr. Suisse d'Electronique et de Microtechnique SA (Switzerland) 5Ecole Polytechnique Fédérale de Lausanne (Switzerland) 6Institut de génétique et de biologie moléculaire et cellulaire (France) 7Univ. de Strasbourg (France) 8Institut National de la Santé et de la Recherche Médicale (France)
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Most of the recent super-resolution imaging techniques developed for biology are based on fluorescence properties which generally require toxic dyes. We show how super-resolution can be obtained without dyes by simply adding 20-micrometer-diameter dielectric spheres on the sample under a microscope objective. The microsphere behaves as a non-classical lens, collecting evanescent waves in a full-field imaging process. Resolutions of up to between /5 and /7 can be reached in air and in immersion, respectively. For translucent biological samples, a dark-field optical setup is proposed. Performance of the label-free super-resolution technique is demonstrated through the imaging of fixed nerve sections from a mouse embryo brain.
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