In vivo high-resolution multimodal nonlinear optical microscopy of spinal cord in mice

Wanjie Wu; Zhongya Qin; Junqiang Wu; Congping Chen; Weitao Chen; Kai Liu; Jianan Y. Qu

doi:10.1117/12.2546925

21 February 2020 In vivo high-resolution multimodal nonlinear optical microscopy of spinal cord in mice

Wanjie Wu, Zhongya Qin, Junqiang Wu, Congping Chen, Weitao Chen, Kai Liu, Jianan Y. Qu

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 112520P (2020) https://doi.org/10.1117/12.2546925
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Chronic in vivo optical imaging of the spinal cord is an effective way to study the biological processes during and after spinal cord injury (SCI) in mouse models. It normally relies on an implanted spinal chamber to provide continuous optical access to the spinal cord. However, the chronic window consists of multiple layers of transparent materials with various optical properties and irregular thickness, which induce large optical aberration. Therefore, the image quality of multiphoton microscopy as well as the precision of femtosecond laser axotomy were dramatically degraded. In this work, we developed an adaptive optics (AO) microscope system integrating stimulated Raman scattering (SRS) and twophoton excited fluorescence (TPEF). Using our system, the aberrations induced by the spinal cord window were measured and compensated accordingly, enabling both high-resolution imaging and precise laser axotomy of the mouse spinal cord.

Citation Download Citation

Wanjie Wu, Zhongya Qin, Junqiang Wu, Congping Chen, Weitao Chen, Kai Liu, and Jianan Y. Qu "In vivo high-resolution multimodal nonlinear optical microscopy of spinal cord in mice", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112520P (21 February 2020); https://doi.org/10.1117/12.2546925

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