Alignment-free frequency modulation stimulated Raman scattering microscopy (Conference Presentation)

Andrew Hill

doi:10.1117/12.2546806

10 March 2020 Alignment-free frequency modulation stimulated Raman scattering microscopy (Conference Presentation)

Andrew Hill

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 112520U (2020) https://doi.org/10.1117/12.2546806
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Stimulated Raman Scattering (SRS) microscopy is a powerful method for imaging molecular distributions based on their intrinsic vibrational contrast. However, SRS is hindered by a parasitic background signal which often overpowers the signal in low-signal applications. Frequency modulation (FM) has been used to suppress this parasitic background. However, many FM-SRS methods require the addition of multiple optomechanical components and extensive realignment. We report a new approach for alignment-free FM-SRS. In conjunction with a parabolically amplified Stokes pulse, we demonstrate near complete background suppression and also utilize the technique to increase the contrast of minority species in heterogeneous biological samples.

Conference Presentation

Citation Download Citation

Andrew Hill "Alignment-free frequency modulation stimulated Raman scattering microscopy (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112520U (10 March 2020); https://doi.org/10.1117/12.2546806

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available