Polarimetric  infrared spectroscopic imaging using quantum cascade lasers

Yamuna Phal; Kevin Yeh; Rohit Bhargava

doi:10.1117/12.2544392

5 March 2020 Polarimetric infrared spectroscopic imaging using quantum cascade lasers

Yamuna Phal, Kevin Yeh, Rohit Bhargava

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 1125210 (2020) https://doi.org/10.1117/12.2544392
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Infrared spectroscopic imaging has emerged as a powerful label-free diagnostic tool to study the molecular composition and organization in biological tissues and cells. We report infrared spectroscopic imaging using polarized light to study differential absorption of plane-polarized light by an oriented sample to detect valuable information, such as, birefringence and dichroism. For instance, the organization of collagen, specifically fiber orientation and alignment, is crucial in understanding the progression and metastasis of cancer. Recent advancements in the development of Quantum Cascade Lasers (QCL) sources have opened new avenues for high SNR measurements in the field of IR spectroscopy. In addition, QCL sources are intrinsically polarized and orientation information can be obtained at discrete frequencies with different polarization orientations, allowing much faster acquisition than a corresponding FT-IR approach. We demonstrate improved performance in terms of fast and comprehensive polarimetric image acquisition and analysis using custom-built QCL microscope and evaluate its impact on applications by analyzing the important spectral bands of surgical tissue sections.

Citation Download Citation

Yamuna Phal, Kevin Yeh, and Rohit Bhargava "Polarimetric infrared spectroscopic imaging using quantum cascade lasers", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125210 (5 March 2020); https://doi.org/10.1117/12.2544392

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available