Hyper-Raman spectroscopy of biologically important molecules

Christopher B. Marble; Xingqi Xu; Kassie S. Marble; Georgi I. Petrov; Dawei Wang; Vladislav V. Yakovlev

doi:10.1117/12.2545215

21 February 2020 Hyper-Raman spectroscopy of biologically important molecules

Christopher B. Marble, Xingqi Xu, Kassie S. Marble, Georgi I. Petrov, Dawei Wang, Vladislav V. Yakovlev

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 112520V (2020) https://doi.org/10.1117/12.2545215
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Raman spectroscopy is an essential optical tool for molecular fingerprints. The vibrational modes of biologically important molecules including proteins, nucleic acids and lipids have been studied to provide insight into their structure as well as insight into the metabolic processes and biomarker expression of cells. To explore hyper-Raman scattering as a complementary technique to Raman scattering, we build a laser system that can perform Raman and hyper-Raman scattering studies using a single setup. Using three amplification stages we are able to generate 8 ps, 1064 nm pulses at repetition rates up to 30 MHz. Converting the 1064 nm source laser to 532 nm, we achieve fast hyper-Raman detection and collect our spectrum with a commercial spectrometer and CCD. Using a single optical setup, we collect and compare Raman spectra at 532 nm to hyper-Raman spectra at 266 nm for water, ethanol and L-tartaric acid. Furthermore, we observe changes in the hyper-Raman peak intensities of an aqueous L-tartaric acid solution when selecting different laser repetition rates highlighting the need to control laser power and repetition rate to identify and mitigate thermal effects in biomolecules.

Citation Download Citation

Christopher B. Marble, Xingqi Xu, Kassie S. Marble, Georgi I. Petrov, Dawei Wang, and Vladislav V. Yakovlev "Hyper-Raman spectroscopy of biologically important molecules", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112520V (21 February 2020); https://doi.org/10.1117/12.2545215

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