Immobilization of HIV GP41 antibodies on glass substrates for HIV biosensing

Sello Manoto; Ahmed El-Hussein; Rudzani Malabi; Lebogang Thobakgale; Saturnin Ombinda-Lemboumba; Yasser Attia; Mohammed Kasem; Patience Mthunzi-Kufa

doi:10.1117/12.2546497

21 February 2020 Immobilization of HIV GP41 antibodies on glass substrates for HIV biosensing

Sello Manoto, Ahmed El-Hussein, Rudzani Malabi, Lebogang Thobakgale, Saturnin Ombinda-Lemboumba, Yasser Attia, Mohammed Kasem, Patience Mthunzi-Kufa

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Proceedings Volume 11258, Frontiers in Biological Detection: From Nanosensors to Systems XII; 112580O (2020) https://doi.org/10.1117/12.2546497
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Biological macromolecules such as antibodies, enzymes, proteins and aptamers have good molecular recognition ability which makes them good candidates for biosensing applications. In this study, glass substrates were treated with silane in order to immobilize HIV gp41 antibodies on their surfaces. The HIV pseudovirus was added to the treated substrates followed by addition of antibodies conjugated to nanoparticles. The surfaces were characterised by using water contact angle, atomic force microscopy (AFM) and Raman spectroscopy. Our preliminary data displayed that the antibodies were indeed immobilized on the glass substrates which made it possible for capturing the intact HIV pseudovirus. Further, Raman spectroscopy revealed the presence of disulphide bonds indicating successful conjugation of the HIV gp41 antibodies to the HIV pseudovirus.

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Sello Manoto, Ahmed El-Hussein, Rudzani Malabi, Lebogang Thobakgale, Saturnin Ombinda-Lemboumba, Yasser Attia, Mohammed Kasem, and Patience Mthunzi-Kufa "Immobilization of HIV GP41 antibodies on glass substrates for HIV biosensing", Proc. SPIE 11258, Frontiers in Biological Detection: From Nanosensors to Systems XII, 112580O (21 February 2020); https://doi.org/10.1117/12.2546497

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