Rajannya Sen,1 Alexander Zhdanov,1 Liisa M. Hirvonen,2 Peter Svihra,3,4 Stefan Andersson-Engels,5 Andrei Nomerotski,6 Dmitri Papkovsky1,5
1Univ. College Cork (Ireland) 2The Univ. of Western Australia (Australia) 3Czech Technical Univ. in Prague (Czech Republic) 4The Univ. of Manchester (United States) 5Tyndall National Institute (Ireland) 6Brookhaven National Lab. (United States)
The recently designed Tpx3Cam camera based PLIM (Phosphorescence Lifetime IMaging) macro-imager was tested using an array of phosphorescent chemical and biological samples. A series of sensor materials prepared by incorporating the phosphorescent O2-sensitive dye, PtBP, into five polymers with different O2 permeability were imaged along with several commercial and non-commercial sensors based on PtBP and PtOEPK dyes. The PLIM images showed good lifetime contrast between the different materials, and phosphorescence lifetime values obtained were consistent with those measured by alternative methods. A panel of live tissues samples stained with PtBP based nanoparticle probe were also prepared and imaged under resting conditions and upon inhibition of respiration. The macro-imager showed promising results as a tool for PLIM of O2 in chemical and biological samples.
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Rajannya Sen, Alexander Zhdanov, Liisa M. Hirvonen, Peter Svihra, Stefan Andersson-Engels, Andrei Nomerotski, Dmitri Papkovsky, "A new macro-imager based on Tpx3Cam optical camera for PLIM applications," Proc. SPIE 11359, Biomedical Spectroscopy, Microscopy, and Imaging, 113591N (1 April 2020); https://doi.org/10.1117/12.2555387