Characterization of auto-fluorescence urine crystals from gout patients using confocal microscopy and micro-Raman system for urolithiasis prediction

Zih-Ting Chen; Chih-Hao Wang; Huihua Kenny Chiang

doi:10.1117/12.2555440

1 April 2020 Characterization of auto-fluorescence urine crystals from gout patients using confocal microscopy and micro-Raman system for urolithiasis prediction

Zih-Ting Chen, Chih-Hao Wang, Huihua Kenny Chiang

Author Affiliations +

Proceedings Volume 11359, Biomedical Spectroscopy, Microscopy, and Imaging; 113591P (2020) https://doi.org/10.1117/12.2555440
Event: SPIE Photonics Europe, 2020, Online Only

Abstract

The major etiologies of gout and kidney stone formation are similar－hyperuremia , which is caused by unbalanced uric acid production/exertion. And gout is one of the important risk factor that promotes kidney stone formation. It’s worth noting that crystals retension/aggregation is the early stage of stone formation. However, the mechanism between gout and crystalluria remains unclear. Our previous studies found good correlation between auto-fluorescent crystals and urolithiasis. Higher proportion of auto-fluorescent crystalluria in gout patients were also noted. It this paper, we demonstrated characterization of auto-fluorescent urinary crystals in gout patients by micro-Raman system and laser confocal microscopy. Our purpose is to figure out the mechanism among gout flare, urolithiasis and auto-fluorescent crystal retention. And the results supported that fluorophore inside the crystals may be a powerful indicator for crystals retention. Further studies are required to validate our hypothesis.

Conference Presentation

Citation Download Citation

Zih-Ting Chen, Chih-Hao Wang, and Huihua Kenny Chiang "Characterization of auto-fluorescence urine crystals from gout patients using confocal microscopy and micro-Raman system for urolithiasis prediction", Proc. SPIE 11359, Biomedical Spectroscopy, Microscopy, and Imaging, 113591P (1 April 2020); https://doi.org/10.1117/12.2555440

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