Polyprotein: a new standard sample to home-built optical tweezers

Shuai Li; Chunguang Hu; Yanhua Ma; Xiaotang Hu

doi:10.1117/12.2539281

12 March 2020 Polyprotein: a new standard sample to home-built optical tweezers

Shuai Li, Chunguang Hu, Yanhua Ma, Xiaotang Hu

Proceedings Volume 11434, 2019 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments; 1143402 (2020) https://doi.org/10.1117/12.2539281
Event: 2019 International Conference on Optical Instruments and Technology, 2019, Beijing, China

Abstract

Optical tweezers is one of the commonly used technologies to research on protein force spectroscopy. However, whether optical tweezers system has the capability of force spectroscopy measurement at the molecular scale is vital to single molecule experiments. In this paper, we test the capability of our home-built dual-trap optical tweezers system by stretching polyprotein (NuG2)₈ which is made of eight identical tandem repeats of NuG2. With the constant velocity stretching and relaxation mode, we achieve a lot of experimental data and get the contour length increment of (NuG2)₈ rapidly from the unfolding processes after fitting these data. The result is consistent with existing reports, which demonstrates optical tweezers system has the force spectroscopy test ability and (NuG2)₈ can be used as a new standard sample to evaluate the test performance of optical tweezers. Using polyprotein (NuG2)₈ as standard sample has two advantages: stretching polyprotein can help improve the efficiency of data statistics and a large number of experiments can reduce the randomness of the system when testing.

Citation Download Citation

Shuai Li, Chunguang Hu, Yanhua Ma, and Xiaotang Hu "Polyprotein: a new standard sample to home-built optical tweezers", Proc. SPIE 11434, 2019 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments, 1143402 (12 March 2020); https://doi.org/10.1117/12.2539281

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