Simulating Traumatic Brain Injury (TBI) using laser-induced shockwave under quantitative phase microscopy

Pegah Pouladian; Toyohiko Yamauchi; Nicole M. Wakida; Michael W. Berns; Daryl Preece

doi:10.1117/12.2569334

20 August 2020 Simulating Traumatic Brain Injury (TBI) using laser-induced shockwave under quantitative phase microscopy

Pegah Pouladian, Toyohiko Yamauchi, Nicole M. Wakida, Michael W. Berns, Daryl Preece

Author Affiliations +

Proceedings Volume 11463, Optical Trapping and Optical Micromanipulation XVII; 1146321 (2020) https://doi.org/10.1117/12.2569334
Event: SPIE Nanoscience + Engineering, 2020, Online Only

Conference Poster

Abstract

Traumatic brain injury (TBI) occurs when an external shock causes injury to the brain. The mechanism of the disease is not completely understood yet. Studies have shown that astrocytes play various roles following brain injury. However, the exact functional role of them after TBI is still a matter of debate. Laser-induced shock waves (LIS) can create a precise controllable mechanical force that is capable of injuring or lysing cells to simulate the brain injury at the cellular level. Here, we propose a system that enables us to induce injuries in CNS cells with LIS and observe the whole process under a Quantitative phase microscope (QPM). Our system is also capable of adding another laser for optically trapping the cells to keep them at a certain distance from the center of the shockwave, as this distance is one of the important factors which determines the level of injury.

Conference Presentation

Citation Download Citation

Pegah Pouladian, Toyohiko Yamauchi, Nicole M. Wakida, Michael W. Berns, and Daryl Preece "Simulating Traumatic Brain Injury (TBI) using laser-induced shockwave under quantitative phase microscopy", Proc. SPIE 11463, Optical Trapping and Optical Micromanipulation XVII, 1146321 (20 August 2020); https://doi.org/10.1117/12.2569334

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