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20 August 2020 Raman imaging of metabolic activities in brain
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Abstract
Brain is a complex organ displaying cellular structural and functional heterogeneity. Understanding the dynamics of metabolism in brain with cellular resolution is essential to unraveling the mechanistic basis of many neuronal activities. Traditional imaging methods cannot provide metabolic activity information with high resolution in situ. We developed a new method that combines bioorthogonal labeling and Raman Scattering microscopy to visualize metabolic dynamics in brains. For example, the incorporation of deuterated glucose, or heavy water (D2O) into biomolecules will generate new carbon-deuterium (C-D) bonds in protein, lipids and nucleic acids in brain. We obtained new insights through high resolution imaging of whole brain, such as new protein and lipids synthesis in different brain regions during the developmental and aging processes. We demonstrate that this is an efficient methods for visualizing metabolic activities in brain.
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Lingyan Shi "Raman imaging of metabolic activities in brain", Proc. SPIE 11497, Ultrafast Nonlinear Imaging and Spectroscopy VIII, 114970S (20 August 2020); https://doi.org/10.1117/12.2571112
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