Label-free imaging of subcellular features with mid-infrared photothermal microscopy

Michelle Y. Sander; Panagis Samolis

doi:10.1117/12.2568744

20 August 2020 Label-free imaging of subcellular features with mid-infrared photothermal microscopy

Michelle Y. Sander, Panagis Samolis

Proceedings Volume 11497, Ultrafast Nonlinear Imaging and Spectroscopy VIII; 114970Y (2020) https://doi.org/10.1117/12.2568744
Event: SPIE Optical Engineering + Applications, 2020, Online Only

Abstract

Mid-infrared photothermal imaging is a novel chemical imaging modality that combines high sensitivity with enhanced spatial resolution. Subcellular features in fibroblast cells and tissues are imaged and analyzed with regards to their molecular structures without the need of exogenous fluorophores at a resolution that overcomes the diffraction limited spot size of the mid-infrared excitation beam. With a phase-sensitive lock-in detection scheme, changes in the thermal diffusion properties can be detected and can provide a complementary sample characterization.

Conference Presentation

Citation Download Citation

Michelle Y. Sander and Panagis Samolis "Label-free imaging of subcellular features with mid-infrared photothermal microscopy", Proc. SPIE 11497, Ultrafast Nonlinear Imaging and Spectroscopy VIII, 114970Y (20 August 2020); https://doi.org/10.1117/12.2568744

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