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20 August 2020 Light-sheet fluorescence microscopy with metalens
Cheng Hung Chu, Yuan Luo, Ming Lun Tseng, Sunil Vyas, Hsin Yu Kuo, Din Ping Tsai, Takuo Tanaka
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Proceedings Volume 11500, ODS 2020: Industrial Optical Devices and Systems; 1150004 (2020) https://doi.org/10.1117/12.2570535
Event: SPIE Optical Engineering + Applications, 2020, Online Only
Abstract
We propose a Light-sheet fluorescent microscopy (LSFM) system with a flat metalens, which is used to replace the bulky illuminating components. The metalens is a diffractive optics elements (DOEs) composed of various gallium nitride (GaN) nanopillar for light-sheet generation, and it can be readily integrated in existing LSFM systems. In contrast to the traditional LSFM system, the metalens-based system is more compact and flexible, and its sectioning capability is comparable to the conventional illumination arm in LSFMs. The live fluorescent-labeled transgenic Caenorhabditis elegans (C. elegans) are exploited to verify the performance of this system. The intensity profile of oocytes in C. elegans with cellular resolution level was obtained.
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Cheng Hung Chu, Yuan Luo, Ming Lun Tseng, Sunil Vyas, Hsin Yu Kuo, Din Ping Tsai, and Takuo Tanaka "Light-sheet fluorescence microscopy with metalens", Proc. SPIE 11500, ODS 2020: Industrial Optical Devices and Systems, 1150004 (20 August 2020); https://doi.org/10.1117/12.2570535
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KEYWORDS
Luminescence
Microscopy
Biomedical optics
Gallium nitride
Integrated optics
Optical components
Spatial resolution
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