Preliminary experimental evaluation of microscopic imaging through thick biological tissues based on in-line phase-shift digital holography using near-infrared light

Manami Ohta; Shutaro Kodama; Kanami Ikeda; Yutaka Kano; Yoko Miyamoto; Wolfgang Osten; Mitsuo Takeda; Eriko Watanabe

doi:10.1117/12.2573228

15 June 2020 Preliminary experimental evaluation of microscopic imaging through thick biological tissues based on in-line phase-shift digital holography using near-infrared light

Manami Ohta, Shutaro Kodama, Kanami Ikeda, Yutaka Kano, Yoko Miyamoto, Wolfgang Osten, Mitsuo Takeda, Eriko Watanabe

Author Affiliations +

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 115210U (2020) https://doi.org/10.1117/12.2573228
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

We previously proposed a three-dimensional microscopic imaging system for objects hidden behind scattering media using in-line phase-shift digital holography, which simultaneously captures amplitude and phase information. However, as the thickness of the scattering medium increases, the influence of scattering is enhanced, and the reconstructed image of the object behind the scattering medium deteriorates. In this paper, we report the evaluation of this image using a near-infrared light source with a wavelength of 780 nm that is capable of deep tissue penetration. A favorable microscopic image of the object behind the rat-skin sample of 912 μm-thickness was successfully reconstructed.

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Manami Ohta, Shutaro Kodama, Kanami Ikeda, Yutaka Kano, Yoko Miyamoto, Wolfgang Osten, Mitsuo Takeda, and Eriko Watanabe "Preliminary experimental evaluation of microscopic imaging through thick biological tissues based on in-line phase-shift digital holography using near-infrared light", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210U (15 June 2020); https://doi.org/10.1117/12.2573228

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