A fluorescence detection optical system for real-time quantitative PCR

Yu-tong Ma; Liang Zeng; Jian-huan Zhang

doi:10.1117/12.2574901

10 October 2020 A fluorescence detection optical system for real-time quantitative PCR

Yu-tong Ma, Liang Zeng, Jian-huan Zhang

Proceedings Volume 11548, Optical Design and Testing X; 115481U (2020) https://doi.org/10.1117/12.2574901
Event: SPIE/COS Photonics Asia, 2020, Online Only

Conference Poster

Abstract

Real-time quantitative PCR technology has been an active filed since it has excellent performance of the ability of quantitative analysis for initial DNA template evaluation and detection on fluorescence signal. This paper basically pay attention on the features of system and optimize the original parts of the device. Using OLED as light source and nonlinear optics device in the light path to optimize the effect of the device. According to simulation results, the uniformity of the excitation in this device has a marked improvement. Furthermore, with the addition of the filed lens, the collection effect of the fluorescence would be better.

Citation Download Citation

Yu-tong Ma, Liang Zeng, and Jian-huan Zhang "A fluorescence detection optical system for real-time quantitative PCR", Proc. SPIE 11548, Optical Design and Testing X, 115481U (10 October 2020); https://doi.org/10.1117/12.2574901

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