Optimal annular illumination pattern for Fourier ptychographic microscopy based on spectrum aliasing minimization

Jiasong Sun; Chao Zuo; Qian Chen

doi:10.1117/12.2573417

10 October 2020 Optimal annular illumination pattern for Fourier ptychographic microscopy based on spectrum aliasing minimization

Jiasong Sun, Chao Zuo, Qian Chen

Author Affiliations +

Proceedings Volume 11549, Advanced Optical Imaging Technologies III; 115490C (2020) https://doi.org/10.1117/12.2573417
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

We present an annular illumination pattern optimization scheme for Fourier ptychographic microscopy (FPM) based on spectrum aliasing minimization. It has been found that an annular illumination light source could provide the highest phase retrieval accuracy while the oblique illumination angles matching the objective NA precisely. However, this conclusion is no longer valid when the Nyquist sampling criterion is not satisfied under the incoherent illumination case. In this paper, we investigate the spectrum aliasing characteristic with different spatial sampling rates. Then, an objective cost function related to the spectrum aliasing percentage and reconstruction error is established to bring about an annular illumination pattern optimization scheme. The reconstruction accuracy improvement of the proposed approach is demonstrated by achieving a full-pitch resolution of 548 nm across a wide FOV of 1.772 mm² while the incoherent spatial sampling criterion is not satisfied.

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Citation Download Citation

Jiasong Sun, Chao Zuo, and Qian Chen "Optimal annular illumination pattern for Fourier ptychographic microscopy based on spectrum aliasing minimization", Proc. SPIE 11549, Advanced Optical Imaging Technologies III, 115490C (10 October 2020); https://doi.org/10.1117/12.2573417

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