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10 October 2020 DMD-addressing based ptychographic phase microscopy
Peng Gao, Ying Ma, Juanjuan Zheng, Kai Wen
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Proceedings Volume 11551, Holography, Diffractive Optics, and Applications X; 115510V (2020) https://doi.org/10.1117/12.2573863
Event: SPIE/COS Photonics Asia, 2020, Online Only
Abstract
This paper presents DMD-addressing based ptychographic phase microscopy (DA-PPM) and phase/fluorescence dual-modality imaging in DA-PPM. Compared with conventional ptychographic approaches, the imaging speed of DA-PPM is significantly enhanced by DMD based illumination selection, and is further enhanced by parallel illumination, i.e., lighting up parallelly multiple sub-areas in one shot. Furthermore, two-folds spatial resolution enhancement can be achieved in DA-PPM by incorporating structured illumination generated by DMD. Last but not least, phase/fluorescence dual-modality imaging will be performed in DA-PPM, providing for the same sample complementary information, including structural and functional information.
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Peng Gao, Ying Ma, Juanjuan Zheng, and Kai Wen "DMD-addressing based ptychographic phase microscopy", Proc. SPIE 11551, Holography, Diffractive Optics, and Applications X, 115510V (10 October 2020); https://doi.org/10.1117/12.2573863
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KEYWORDS
Microscopy
Digital micromirror devices
CCD cameras
Diffraction
Light sources and illumination
Live cell imaging
Micromirrors
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