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10 October 2020 Structured illumination microscopy using digital micromirror device and coherent light source
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Abstract
Structured illumination microscopy (SIM) achieves doubled spatial resolution through exciting the specimen with high-contrast, high-frequency sinusoidal patterns. Such an illumination pattern can be generated by laser interference or incoherent structured pattern. Opto-electronic devices, such as Spatial Light Modulator (SLM) or Digital Micro-mirror Device (DMD), can provide rapid switch of illumination patterns for SIM. Although DMD is much more cost-effective than SLM, it was previously restricted in association with incoherent light sources. To extend its application with coherent illumination, we model the DMD as a blazed grating, and simulate the effect with DMD pattern changes in SIM. Based on the simulation, we report a fast, high-resolution and cost-efficient SIM with DMD. Our home-built laser interference-based DMD-SIM (LiDMD-SIM) reveals the nuclear pore complex and microtubule in mammalian cells with doubled spatial resolution.
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Meiqi Li, Yaning Li, Wenhui Liu, Amit Lal, Shan Jiang, Dayong Jin, Houpu Yang, Shu Wang, Karl Zhanghao, and Peng Xi "Structured illumination microscopy using digital micromirror device and coherent light source", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 1155313 (10 October 2020); https://doi.org/10.1117/12.2575154
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