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10 October 2020 A surface-enhanced Raman scattering molecular beacon for the detection of miRNA-21
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Abstract
A surface-enhanced Raman scattering (SERS) based microRNA (miRNA) probe was designed using molecular beacon (MB). In such a probe, silver nanoparticles were employed as the SERS substrate while 6-FAM was selected as a Raman reporter, which were connected by a hairpin shaped DNA. In the presence of target miRNA, the hairpin opened, making 6-FAM far away from the substrate with a decreased SERS intensity. As the concentration of the targets increased, the SERS intensity of Raman reporters decreased. Thus, according to the intensity-concentration calibration curve, the detection of miRNA-21 could be realized. The convenient, stable and sensitive detection method holds potential in the application of diagnosis.
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© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Zhile Wang, Kai Zhu, Shenfei Zong, Zhuyuan Wang, and Yiping Cui "A surface-enhanced Raman scattering molecular beacon for the detection of miRNA-21", Proc. SPIE 11554, Advanced Sensor Systems and Applications X, 115541Q (10 October 2020); https://doi.org/10.1117/12.2585146
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