Planar light-sheets from curved laser beams for two-photon microscopy

Tom Vettenburg

doi:10.1117/12.2584561

4 October 2020 Planar light-sheets from curved laser beams for two-photon microscopy

Tom Vettenburg

Proceedings Volume 11575, Biophotonics and Biomedical Microscopy; 115750B (2020) https://doi.org/10.1117/12.2584561
Event: SPIE Photonex and Vacuum Expo, 2020, Online Only

Abstract

Light-sheet fluorescence microscopy allows minimally invasive 3D imaging of biological samples. Unlike other microscopes, a light-sheet illuminates the focal plane from the side. To uniformly illuminate a wide field-of-view, propagation-invariant light-fields have been proposed. While the propagation-invariant Airy beam is particularly advantageous for single-photon excitation, its multi-photon performance is limited by its intricate transversal structure and curvature. Here we demonstrate the first planar Airy beam light-sheet. Its uniform and symmetric illumination enables rapid two-photon excitation across a wide field-of-view. Moreover, it eliminates the need for deconvolution and it can significantly simplify a dual-use, single and two-photon, imaging instrument.

Conference Presentation

Citation Download Citation

Tom Vettenburg "Planar light-sheets from curved laser beams for two-photon microscopy", Proc. SPIE 11575, Biophotonics and Biomedical Microscopy, 115750B (4 October 2020); https://doi.org/10.1117/12.2584561

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