Mapping out lesion heterogeneity on (sub)cm scale with a fast large area multiphoton exoscope (FLAME)

Alexander Fast; Griffin Lentsch; Amanda Durkin; Christopher Zachary M.D.; Kristen Kelly M.D.; Mihaela Balu; Linda Doan M.D.; Bonnie Lee M.D.

doi:10.1117/12.2579260

5 March 2021 Mapping out lesion heterogeneity on (sub)cm scale with a fast large area multiphoton exoscope (FLAME)

Alexander Fast, Griffin Lentsch, Amanda Durkin, Christopher Zachary M.D., Kristen Kelly M.D., Mihaela Balu, Linda Doan M.D., Bonnie Lee M.D.

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Proceedings Volume 11618, Photonics in Dermatology and Plastic Surgery 2021; 116180X (2021) https://doi.org/10.1117/12.2579260
Event: SPIE BiOS, 2021, Online Only

Abstract

Multiphoton microscopy can provide sub-micron resolution images of living tissues in their native environment with chemical contrast. We recently reported on a fast large area multiphoton exoscope (FLAME) for rapidly mapping out macroscopic tissue areas (cm-scale) with microscopic resolution. In this presentation we demonstrate the imaging capability and the clinical utility of this system by performing a pilot study on ex vivo imaging of benign and malignant pigmented lesions of human skin. We identify morphological features such as cytological atypia, lentiginous hyperplasia, migration of melanocytes and demonstrate the value of sampling large tissue volumes for capturing the lesion heterogeneity.

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Alexander Fast, Griffin Lentsch, Amanda Durkin, Christopher Zachary M.D., Kristen Kelly M.D., Mihaela Balu, Linda Doan M.D., and Bonnie Lee M.D. "Mapping out lesion heterogeneity on (sub)cm scale with a fast large area multiphoton exoscope (FLAME)", Proc. SPIE 11618, Photonics in Dermatology and Plastic Surgery 2021, 116180X (5 March 2021); https://doi.org/10.1117/12.2579260

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KEYWORDS

Skin

Associative arrays

In vivo imaging

Chemical elements

Convolutional neural networks

Denoising

Diagnostics

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