Line-scanning optical sectioning by digital structured modulation

Rui Jin; Qiuyuan Zhong; Chenyu Jiang; Dejie Zhang; Siqi Chen; Hui Gong; Jing Yuan

doi:10.1117/12.2582890

5 March 2021 Line-scanning optical sectioning by digital structured modulation

Rui Jin, Qiuyuan Zhong, Chenyu Jiang, Dejie Zhang, Siqi Chen, Hui Gong, Jing Yuan

Author Affiliations +

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116290K (2021) https://doi.org/10.1117/12.2582890
Event: SPIE BiOS, 2021, Online Only

Abstract

Block face imaging is widely used in three-dimensional large biological samples imaging. However, high throughput and excellent optical sectioning cannot be achieved at the same time for imaging such large area. Here, we propose a line-scanning virtual structured modulation method to combine the advantages of optical sectioning and imaging speed in structured modulation microscopy and line-scanning microscopy. We significantly improve signal to noise ratio and throughput compared to wild-filed structured illumination microscopy. Our results also have no residual modulation artifacts. It indicates our method enables to achieve imaging 3D large biological sample with excellent optical sectioning and high throughput.

Conference Presentation

Citation Download Citation

Rui Jin, Qiuyuan Zhong, Chenyu Jiang, Dejie Zhang, Siqi Chen, Hui Gong, and Jing Yuan "Line-scanning optical sectioning by digital structured modulation", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116290K (5 March 2021); https://doi.org/10.1117/12.2582890

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