Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy

Wenjun Shao; Kivilcim Kilic; Wenqing Yin; Gregory Wirak; Xiaodan Qin; Hui Feng; David Boas; Christopher V. Gabel; Ji Yi

doi:10.1117/12.2577414

5 March 2021 Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy

Wenjun Shao, Kivilcim Kilic, Wenqing Yin, Gregory Wirak, Xiaodan Qin, Hui Feng, David Boas, Christopher V. Gabel, Ji Yi

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116291V (2021) https://doi.org/10.1117/12.2577414
Event: SPIE BiOS, 2021, Online Only

Abstract

Most reports on oblique plane microscopy (OPM) have a limited microscopic field of view (FOV), typically within 1x1 mm2. We implemented an optical design of mesoscopic scanning oblique plane microscopy (OPM) to allow using low numerical aperture (NA) objective lens. The angle of the intermediate image before the remote focusing system was increased by a demagnification under Scheimpflug condition such that the light collecting efficiency in the remote focusing system was significantly improved. We demonstrate a mesoscopic FOV up to ~6× 5×0.6 mm3 volumetric imaging, the largest reported FOV by OPM so far.

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Wenjun Shao, Kivilcim Kilic, Wenqing Yin, Gregory Wirak, Xiaodan Qin, Hui Feng, David Boas, Christopher V. Gabel, and Ji Yi "Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116291V (5 March 2021); https://doi.org/10.1117/12.2577414

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KEYWORDS

Objectives

Microscopy

3D visualizations

Image resolution

Luminescence

Neurons

Optical design

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