Applications of dynamic micro-optical coherence tomography

Hui Min Leung; Linhui Yu; Devon M. Thompson; Yi Sun; Abigail L. Gregg; Joseph A. Gardecki; Russell W. Jenkins; Oliver Jonas; Guillermo J. Tearney

doi:10.1117/12.2583649

5 March 2021 Applications of dynamic micro-optical coherence tomography

Hui Min Leung, Linhui Yu, Devon M. Thompson, Yi Sun, Abigail L. Gregg, Joseph A. Gardecki, Russell W. Jenkins, Oliver Jonas, Guillermo J. Tearney

Author Affiliations +

Proceedings Volume 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV; 116301A (2021) https://doi.org/10.1117/12.2583649
Event: SPIE BiOS, 2021, Online Only

Abstract

Living cells exhibit active intracellular molecular motion that reflect their functional states. Traditional microscopy techniques that solely capture high resolution static images of cells misses out on the opportunity to tap into the wealth of information provided by complex intracellular activity. We recently developed dynamic micro-OCT (DμOCT), an extension of μOCT that achieves near-isotropic sub-cellular resolution in all three dimensions (2 µm lateral x 1 µm axial). DμOCT substantially enhanced the contrast of cells and organelles while revealing stratified, depth-dependent dynamics in the epithelial layers. In this work, we have expanded the application DμOCT to encompass imaging of human skin in vivo, and detection of pharmacologic-induced changes in human melanoma spheroids and murine tissues.

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Hui Min Leung, Linhui Yu, Devon M. Thompson, Yi Sun, Abigail L. Gregg, Joseph A. Gardecki, Russell W. Jenkins, Oliver Jonas, and Guillermo J. Tearney "Applications of dynamic micro-optical coherence tomography", Proc. SPIE 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV, 116301A (5 March 2021); https://doi.org/10.1117/12.2583649

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