Biophysical properties of human cells probed with fluidic assays and digital holographic microscopy

Erik Bélanger; Émile Rioux; Corentin Soubeiran; Marie-Ève Crochetière; Jean-Xavier Giroux; Pierre Marquet

doi:10.1117/12.2577402

5 March 2021 Biophysical properties of human cells probed with fluidic assays and digital holographic microscopy

Erik Bélanger, Émile Rioux, Corentin Soubeiran, Marie-Ève Crochetière, Jean-Xavier Giroux, Pierre Marquet

Proceedings Volume 11637, Microfluidics, BioMEMS, and Medical Microsystems XIX; 116370K (2021) https://doi.org/10.1117/12.2577402
Event: SPIE BiOS, 2021, Online Only

Abstract

Recently, interest in the biophysics of cells has been stimulated by evidence from many studies that external force applied to a cell generates signals that are as potent as those of biochemical stimuli for cell growth, differentiation, migration and function. Furthermore, living cells as open systems maintain their homeostasis, i.e. the internal condition necessary for physiological functioning, by exchanging substances with their environments including energy substrate, ions and water across their membrane. Consequently, the objective is to develop milli/microfluidic assays to measure biophysical properties of human cells with a multi-modality imaging system combining digital holographic microscopy and fluorescence microscopy.

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Citation Download Citation

Erik Bélanger, Émile Rioux, Corentin Soubeiran, Marie-Ève Crochetière, Jean-Xavier Giroux, and Pierre Marquet "Biophysical properties of human cells probed with fluidic assays and digital holographic microscopy", Proc. SPIE 11637, Microfluidics, BioMEMS, and Medical Microsystems XIX, 116370K (5 March 2021); https://doi.org/10.1117/12.2577402

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KEYWORDS

Digital holography

Microscopy

Holography

Microfluidics

Imaging devices

Refractive index

Refractometry

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