Delineating breast cancer margins using multimodal optical imaging

Androniki Mitrou; Xin Feng; Ashraf Khan; Anna N. Yaroslavsky

doi:10.1117/12.2588575

5 March 2021 Delineating breast cancer margins using multimodal optical imaging

Androniki Mitrou, Xin Feng, Ashraf Khan, Anna N. Yaroslavsky

Proceedings Volume 11641, Dynamics and Fluctuations in Biomedical Photonics XVIII; 1164103 (2021) https://doi.org/10.1117/12.2588575
Event: SPIE BiOS, 2021, Online Only

Abstract

Quick, macroscopic examination of breast tissue from surgical excisions or biopsies can guide breast cancer patient management. To this purpose, we examined dual-contrast fluorescence imaging with intravital dye Methylene Blue (MB) and disease-specific molecular marker pH low insertion peptide (pHLIP) conjugated with fluorescent Alexa532 (Alexa532-pHLIP) as contrast agents. Samples were stained with both MB and Alexa532-pHLIP and imaged with multimodal wide-field system. Co- and cross-polarized reflectance and fluorescence images were acquired. Then, specimens were processed for H and E paraffin embedded histopathology. Wide-field imaging demonstrated increased pHLIP-Alexa 532 fluorescence emission and high MB fluorescence polarization in cancerous regions.

Conference Presentation

Citation Download Citation

Androniki Mitrou, Xin Feng, Ashraf Khan, and Anna N. Yaroslavsky "Delineating breast cancer margins using multimodal optical imaging", Proc. SPIE 11641, Dynamics and Fluctuations in Biomedical Photonics XVIII, 1164103 (5 March 2021); https://doi.org/10.1117/12.2588575

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