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5 March 2021 Label-free virtual H and E histopathology using f-mode ultraviolet photoacoustic remote sensing
Pradyumna Kedarisetti, Nathaniel J. M. Haven, Brendon S. Restall, Matthew T. Martell, Roger J. Zemp
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Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 116422S (2021) https://doi.org/10.1117/12.2578545
Event: SPIE BiOS, 2021, Online Only
Abstract
We report the use of frequency decomposition with ultraviolet photoacoustic remote sensing (UV-PARS) microscopy that enables contact-free and label-free virtual H and E staining. Along with principal component analysis of frequency information, this image reconstruction technique allows for the incorporation of scale-specific information encoded in the frequency spectrum of generated PARS signals. The performance of this image reconstruction technique as demonstrated on histological samples achieves contrast-to-noise ratios of over 55-dB between nuclei and nearby cytoplasmic structures. This technique allows for the rapid and facile production of virtual H and E stained images without the need for additional excitation wavelengths.
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Pradyumna Kedarisetti, Nathaniel J. M. Haven, Brendon S. Restall, Matthew T. Martell, and Roger J. Zemp "Label-free virtual H and E histopathology using f-mode ultraviolet photoacoustic remote sensing", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116422S (5 March 2021); https://doi.org/10.1117/12.2578545
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KEYWORDS
Photoacoustic spectroscopy
Remote sensing
Ultraviolet radiation
Image restoration
Electronic filtering
Microscopy
Photoacoustic microscopy
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