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5 March 2021 Label-free virtual H and E histopathology using f-mode ultraviolet photoacoustic remote sensing
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Abstract
We report the use of frequency decomposition with ultraviolet photoacoustic remote sensing (UV-PARS) microscopy that enables contact-free and label-free virtual H and E staining. Along with principal component analysis of frequency information, this image reconstruction technique allows for the incorporation of scale-specific information encoded in the frequency spectrum of generated PARS signals. The performance of this image reconstruction technique as demonstrated on histological samples achieves contrast-to-noise ratios of over 55-dB between nuclei and nearby cytoplasmic structures. This technique allows for the rapid and facile production of virtual H and E stained images without the need for additional excitation wavelengths.
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© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Pradyumna Kedarisetti, Nathaniel J. M. Haven, Brendon S. Restall, Matthew T. Martell, and Roger J. Zemp "Label-free virtual H and E histopathology using f-mode ultraviolet photoacoustic remote sensing", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116422S (5 March 2021); https://doi.org/10.1117/12.2578545
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