Photoacoustic remote sensing 3D H&E histology with fluorescence validation

Brendon S. Restall; Nathaniel J. M. Haven; Matthew T. Martell; Pradyumna Kedarisetti; Brendyn Cikaluk; Lashan Peiris; Sveta Silverman; Jean Deschenes; Roger J. Zemp

doi:10.1117/12.2578944

5 March 2021 Photoacoustic remote sensing 3D H and E histology with fluorescence validation

Brendon S. Restall, Nathaniel J. M. Haven, Matthew T. Martell, Pradyumna Kedarisetti, Brendyn Cikaluk, Lashan Peiris, Sveta Silverman, Jean Deschenes, Roger J. Zemp

Author Affiliations +

Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 1164231 (2021) https://doi.org/10.1117/12.2578944
Event: SPIE BiOS, 2021, Online Only

Abstract

Hematoxylin and Eosin (H and E) staining is the gold standard for the majority of histopathological diagnostics but requires lengthy processing times not suitable for point-of-care diagnosis. Here we demonstrate a 266-nm excitation Ultraviolet Photoacoustic Remote Sensing (UV-PARS) and Scattering Microscopy system capable of virtual H and E 3D imaging of tissues in conjunction with with confocal fluorescence microscopy (CFM) for validation in thick tissues. We demonstrate the capabilities of this dual-contrast system for en-face planar and volumetric imaging of human tissue samples exhibiting high concordance with the gold standard of H and E staining procedures as well as confocal fluorescence microscopy. To our knowledge, this is the first near real-time microscopy approach capable of volumetric imaging unstained thick tissues with virtual H and E contrast.

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Brendon S. Restall, Nathaniel J. M. Haven, Matthew T. Martell, Pradyumna Kedarisetti, Brendyn Cikaluk, Lashan Peiris, Sveta Silverman, Jean Deschenes, and Roger J. Zemp "Photoacoustic remote sensing 3D H and E histology with fluorescence validation", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 1164231 (5 March 2021); https://doi.org/10.1117/12.2578944

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