Comparison of transmission- and reflectance-mode quantitative polarized light imaging (QPLI) for microstructural analysis of collagenous soft tissues

Leanne Iannucci; Viktor Gruev; Spencer P. Lake

doi:10.1117/12.2576709

5 March 2021 Comparison of transmission- and reflectance-mode quantitative polarized light imaging (QPLI) for microstructural analysis of collagenous soft tissues

Leanne Iannucci, Viktor Gruev, Spencer P. Lake

Author Affiliations +

Proceedings Volume 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics; 1164609 (2021) https://doi.org/10.1117/12.2576709
Event: SPIE BiOS, 2021, Online Only

Abstract

Biomechanical function of musculoskeletal soft tissues is dictated by their hierarchically organized collagen extracellular matrices. Our lab has used quantitative polarized light imaging (QPLI) to evaluate dynamic collagen fiber alignment in soft tissues. However, thinning of tissue for light transmission precludes the use of QPLI in more physiologically relevant scenarios. Reflectance mode QPLI could allow for in situ analysis of collagen microstructure. In this study, signal obtained via reflectance and transmission mode QPLI of engineered soft tissue analogs with prescribed collagen fiber alignment were compared in order to understand differences between modes and to provide context for further orthopedic applications.

Conference Presentation

Citation Download Citation

Leanne Iannucci, Viktor Gruev, and Spencer P. Lake "Comparison of transmission- and reflectance-mode quantitative polarized light imaging (QPLI) for microstructural analysis of collagenous soft tissues", Proc. SPIE 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics, 1164609 (5 March 2021); https://doi.org/10.1117/12.2576709

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