Polarization and phase imaging using an LED array microscope

Xiang Dai; Pavan Chandra Konda; Shiqi Xu; Roarke Horstmeyer

doi:10.1117/12.2577344

5 March 2021 Polarization and phase imaging using an LED array microscope

Xiang Dai, Pavan Chandra Konda, Shiqi Xu, Roarke Horstmeyer

Proceedings Volume 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics; 116460U (2021) https://doi.org/10.1117/12.2577344
Event: SPIE BiOS, 2021, Online Only

Abstract

We present a simple low-cost microscope that uses a vectorial extension of Fourier ptychography to recover the absorption, phase and polarization properties of a sample at high-NA across a wide field-of-view. Our principle is validated by experimentally imaging quantitative test targets as well as a plant root and rabbit spinal cord cross-sections, with which we demonstrate the ability to record complex specimen birefringence over 10.4 mm2 field-of-view at 0.73um resolution. Our new Fourier ptychographic approach also enables the measurement and correction of polarization-dependent pupil aberrations. We hope this simplicity helps adapt joint polarization and phase imaging to a wider array of applications.

Conference Presentation

Citation Download Citation

Xiang Dai, Pavan Chandra Konda, Shiqi Xu, and Roarke Horstmeyer "Polarization and phase imaging using an LED array microscope", Proc. SPIE 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics, 116460U (5 March 2021); https://doi.org/10.1117/12.2577344

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