Label-free optical detection of circulating tumor cell clusters using confocal back scatter and fluorescence flow cytometry

Nilay Vora; Jasmine Kwan; Andrew Wishart; Madeleine Oudin; Irene Georgakoudi

doi:10.1117/12.2578013

5 March 2021 Label-free optical detection of circulating tumor cell clusters using confocal back scatter and fluorescence flow cytometry

Nilay Vora, Jasmine Kwan, Andrew Wishart, Madeleine Oudin, Irene Georgakoudi

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Proceedings Volume 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX; 116470S (2021) https://doi.org/10.1117/12.2578013
Event: SPIE BiOS, 2021, Online Only

Abstract

Rare circulating tumor cells (CTCs) and circulating tumor cells clusters (CTCCs) have been shown to increase the metastatic potential of tumors, with CTCCs increasing metastatic potential by 23 to 50 times. Due to the high metastatic potential of CTCCs, there is a growing interest in the detection and isolation of these clusters to improve diagnosis, stratification, and treatment of cancer patients. This study aims to utilize a custom confocal back scatter and fluorescence flow cytometer (CBSFFC) that will leverage the high sensitivity of flow cytometers for in vitro and in vivo detection of CTCCs without the use of exogenous contrast agents.

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Nilay Vora, Jasmine Kwan, Andrew Wishart, Madeleine Oudin, and Irene Georgakoudi "Label-free optical detection of circulating tumor cell clusters using confocal back scatter and fluorescence flow cytometry", Proc. SPIE 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX, 116470S (5 March 2021); https://doi.org/10.1117/12.2578013

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KEYWORDS

Tumors

Confocal microscopy

Luminescence

Flow cytometry

Blood

In vitro testing

Signal to noise ratio

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