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5 March 2021 Multiphoton autofluorescence imaging of immune cells in cancer
Melissa C. Skala, Tiffany M. Heaster, Kelsey Tweed, Alexa Heaton, Dan Pham, Katherine Mueller, Dave Beebe, Paul Sondel, Krishanu Saha
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Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 1164807 (2021) https://doi.org/10.1117/12.2579252
Event: SPIE BiOS, 2021, Online Only
Abstract
Current methods to assess immune cell function use labels that are limiting for time-course studies of immune cell behavior in tumors. Here, we use multiphoton imaging of NAD(P)H and FAD, co-enzymes of metabolism, in T cells and macrophages within the tumor microenvironment. T cells alter their metabolism in response to tumor-like pH, glucose, and lactic acid levels, while macrophages alter their metabolism during tumor-stimulated migration. These results indicate that multiphoton autofluorescence imaging is a powerful label-free method to monitor immune cell metabolism within single cells in the tumor microenvironment.
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Melissa C. Skala, Tiffany M. Heaster, Kelsey Tweed, Alexa Heaton, Dan Pham, Katherine Mueller, Dave Beebe, Paul Sondel, and Krishanu Saha "Multiphoton autofluorescence imaging of immune cells in cancer", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 1164807 (5 March 2021); https://doi.org/10.1117/12.2579252
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KEYWORDS
Tumors
Cancer
Auto-fluorescence imaging
Mode conditioning cables
3D modeling
Mouse models
Multiphoton microscopy
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