Microfluid setup for high throughput laser-assisted cell transfection

Hans Georg Breunig; Karsten König

doi:10.1117/12.2577554

5 March 2021 Microfluid setup for high throughput laser-assisted cell transfection

Hans Georg Breunig, Karsten König

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480P (2021) https://doi.org/10.1117/12.2577554
Event: SPIE BiOS, 2021, Online Only

Abstract

By the interaction with femtosecond-laser pulses, a cell membrane can be made transiently permeable for macro molecules which enables cell transfection and cell reprogramming. While adherent cells can be effectively targeted subsequently with high precision, we have previously shown that the realization of a continuous flow of cells through a laser focus region increases the number of addressable cells tremendously, although, at the cost of precise individual cell targeting. We describe, here, our improved new setup, which employs microfluidic-flow control and online monitoring of the flow using a high-speed camera, and steps towards optimizing the flow and laser parameters. The capabilities of the setup, which combines femtosecond-laser excitation, control of microfluidic cell flow, and high-speed imaging, are illustrated.

Conference Presentation

Citation Download Citation

Hans Georg Breunig and Karsten König "Microfluid setup for high throughput laser-assisted cell transfection", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480P (5 March 2021); https://doi.org/10.1117/12.2577554

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