Simultaneous multimodal optical coherence and three-photon microscopy of the mouse brain in the 1700 nm optical window in vivo

Xusan Yang; Siyang Liu; Fei Xia; Meiqi Wu; Steven Adie; Chris Xu

doi:10.1117/12.2582750

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5 March 2021 Simultaneous multimodal optical coherence and three-photon microscopy of the mouse brain in the 1700 nm optical window in vivo

Xusan Yang, Siyang Liu, Fei Xia, Meiqi Wu, Steven Adie, Chris Xu

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Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 1164817 (2021) https://doi.org/10.1117/12.2582750
Event: SPIE BiOS, 2021, Online Only

Abstract

Multimodal imaging combining various imaging approaches can provide complementary information about tissue in a single imaging session. Here, we introduce a multimodal approach combining three-photon microscopy (3PM) and spectral-domain optical coherence microscopy (SD-OCM) for the first time. We demonstrate the use of an optical parametric chirped-pulse amplification (OPCPA) laser source, commonly used for three-photon fluorescence excitation and thirdharmonic generation (THG), for simultaneous OCM and three-photon (3P) imaging. We validated the system in deep mouse brain in vivo with neuronal imaging. We visualized small structures such as myelinated axons, neurons, and large fiber tracts in white matter with high spatial resolution in a fast and non-invasive manner using linear and nonlinear contrast in the deep mouse brain (>1 mm) with an OPCPA source operating at 1620 nm central wavelength. Our method demonstrates the potential of the system for simultaneous OCM and 3PM with the same laser source.

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Xusan Yang, Siyang Liu, Fei Xia, Meiqi Wu, Steven Adie, and Chris Xu "Simultaneous multimodal optical coherence and three-photon microscopy of the mouse brain in the 1700 nm optical window in vivo", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 1164817 (5 March 2021); https://doi.org/10.1117/12.2582750

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