High-throughput 3D imaging with compressive lens-free tomography

Zhenxiang Luo; Abdulkadir Yurt; Murali Jayapala; Andy Lambrechts; Dries Braeken; Liesbet Lagae

doi:10.1117/12.2578103

5 March 2021 High-throughput 3D imaging with compressive lens-free tomography

Zhenxiang Luo, Abdulkadir Yurt, Murali Jayapala, Andy Lambrechts, Dries Braeken, Liesbet Lagae

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490N (2021) https://doi.org/10.1117/12.2578103
Event: SPIE BiOS, 2021, Online Only

Abstract

We report a fast and effective lens-free imaging platform for optical diffraction tomography (ODT). Using single wavelength illumination from only 4 angular directions and a lensless inline holographic imaging setup to directly capture the resulting diffraction patterns, our method can reconstruct high quality 3D images of biological samples at micron-scale resolution across a cubic-millimeter-level volume with a compact, scalable and inexpensive system. To achieve this, we developed a compressive tomographic reconstruction algorithm to solve the inverse problem of lens-free ODT by combining Wirtinger derivatives and primal-dual splitting. This fast and inexpensive lens-free tomographic microscopy system provides a promising 3D imaging approach for high throughput biomedical applications.

Conference Presentation

Citation Download Citation

Zhenxiang Luo, Abdulkadir Yurt, Murali Jayapala, Andy Lambrechts, Dries Braeken, and Liesbet Lagae "High-throughput 3D imaging with compressive lens-free tomography", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490N (5 March 2021); https://doi.org/10.1117/12.2578103

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